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. 2013 Nov;57(11):5737-9.
doi: 10.1128/AAC.01433-13. Epub 2013 Aug 26.

New structure of phage-related islands carrying fusB and a virulence gene in fusidic acid-resistant Staphylococcus epidermidis

Hsiao-Jan Chen  1 , Ya-Chun Chang, Jui-Chang Tsai, Wei-Chun Hung, Yu-Tzu Lin, Shang-Jie You, Sung-Pin Tseng, Lee-Jene Teng
Affiliations

New structure of phage-related islands carrying fusB and a virulence gene in fusidic acid-resistant Staphylococcus epidermidis

Hsiao-Jan Chen et al. Antimicrob Agents Chemother. 2013 Nov.

Abstract

Nucleotide sequencing of the fusB-flanking regions in two fusidic acid-resistant Staphylococcus epidermidis isolates with the type IV aj1-leader peptide (LP)-fusB structure (lacking aj1) revealed that their fusB gene was located on novel phage-related islands inserted downstream of smpB and are here referred to as SeRIfusB-3692 and SePIfusB-857. The novel SePIfusB-857 structure was followed by SeCI857, forming a composite pathogenicity island which contained a putative virulence gene, vapE. The linkage of fusB and vapE may contribute to bacterial adaption.

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Figures

Fig 1
Fig 1
Genetic organization of SeRIfusB-3692 (GenBank accession no. AB828059) and SeRIfusB-857 (GenBank accession no. AB828060) compared with those of SeRIfusB-7778 (GenBank accession no. JF808726), partial SeRIfusB-704 (GenBank accession no. JF808725), S. epidermidis 14.1.R1.SE (GenBank accession no. AGUC01000114), and the PI in S. epidermidis FR1909 (GenBank accession no. AENR01000001 and AENR01000008). Genes are drawn according to their sequences and function. ▨, int and xis; ▩, transcription regulators; ▤, replication genes (including the primase gene[pri] and the replication initiator gene [rep]); ▩, the replication origin (ori); formula image, the terminase small-subunit encapsidation gene terS; formula image, other encapsidation genes; formula image, pif (phage interference); ■, aj1-LP-fusB regions; formula image, vapE; formula image, virulence factor genes; formula image, transposase genes; □, chromosome genes adjacent to SeRIfusB; formula image, other genes coding hypothetical proteins. The predicted direct repeats are indicated by vertical arrows. The horizontal arrows represent the PCR primers used to determine the insertion sites (downstream of smpB). Homologous regions between resistance islands are shown with shaded connecting lines, and shaded numbers show the percentages of homology between the corresponding sequences. DR, direct repeat; S. epi, S. epidermidis; ssra, SsrA-binding protein encoded by smpB.
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References

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