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Comparative Study
. 2013:20:27.
doi: 10.1051/parasite/2013027. Epub 2013 Aug 29.

Schistosoma mansoni experimental infection in Mus spretus (SPRET/EiJ strain) mice

Luis Pérez del Villar  1 , Belén Vicente, Purificación Galindo-Villardón, Andrés Castellanos, Jesús Pérez-Losada, Antonio Muro
Affiliations
Comparative Study

Schistosoma mansoni experimental infection in Mus spretus (SPRET/EiJ strain) mice

Luis Pérez del Villar et al. Parasite. 2013.

Abstract
in English, French

Most Schistosoma mansoni experimental infections are developed in several inbred strains of Mus musculus as definitive host. In contrast, Mus spretus is unexplored in Schistosoma infection studies. Mus spretus provides a high variation of immunological phenotypes being an invaluable tool for genetic studies and gene mapping. The aim of this study is to characterize hematological and immunological responses against Schistosoma mansoni infection in Mus spretus (SPRET/EiJ strain) vs. Mus musculus (CD1 strain) mice. Nine weeks after cercarial exposure, animals were perfused and the parasite burden was assessed. The parasitological data suggests that SPRET/EiJ mice tolerate higher parasite loads compared to CD1 strain. In addition, hematological parameters measured in Mus spretus group showed a significant increase in granulocytes population in early stages of infection compared to the CD1 cohort. Meanwhile, CD1 presented higher levels of lymphocytes and IgG1 in the late stages of S. mansoni experimental infection.

La plupart des infections expérimentales à Schistosoma mansoni sont développées chez Mus musculus comme hôte définitif. Au contraire, Mus spretus est une espèce de souris inexplorée dans les infections expérimentales á Schistosoma. Mus spretus offre une grande variation de phénotypes immunologiques, ce qui est un outil essentiel pour les études génétiques et la cartographie des gènes. L’objectif de cette étude est la caractérisation de la réponse hématologique et immunologique contre l’infection à Schistosoma mansoni chez Mus spretus (souche SPRET/EiJ) comparée à Mus musculus (souche CD1). Neuf semaines après l’exposition aux cercaires, les animaux ont été perfusés et les paramètres parasitologiques ont été obtenus. Les données parasitologiques suggèrent que la souche SPRET/EiJ tolère des charges parasitaires plus élevées que la souche CD1. Les paramètres hématologiques mesurés chez SPRET/EiJ ont montré aussi une augmentation significative de la population des granulocytes dans les premiers stades de l’infection par comparaison à la cohorte CD1. Cependant, la souche CD1 a présenté des niveaux plus élevés de lymphocytes et IgG1 dans les stades tardifs de l’infection expérimentale à S. mansoni.

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Figures

Figure 1.
Figure 1.
Boxplot graph displaying the levels of IgG2a (A) and IgG1 (B) along Schistosoma mansoni infection, according to sex and strain of mice. +p < 0.05.
See this image and copyright information in PMC

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