Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Oct;10(10):5529-31.
doi: 10.1128/mcb.10.10.5529-5531.1990.

A 22-amino-acid peptide restores DNA-binding activity to dimerization-defective mutants of the estrogen receptor

J A Lees  1 , S E Fawell, R White, M G Parker
Affiliations

A 22-amino-acid peptide restores DNA-binding activity to dimerization-defective mutants of the estrogen receptor

J A Lees et al. Mol Cell Biol. 1990 Oct.

Abstract

We have identified residues within the estrogen receptor that are required for dimerization and high-affinity DNA binding. A 22-amino-acid peptide encompassing these residues was sufficient to restore DNA-binding activity to a mutant receptor lacking most of the hormone-binding domain. Point mutagenesis of the fusion protein confirmed that this sequence continued to mediate dimerization in a manner similar to that within the native receptor, although its position relative to the DNA-binding domain was appreciably altered.

PubMed Disclaimer

References

    1. EMBO J. 1986 May;5(5):891-7 - PubMed
    1. J Mol Biol. 1980 Mar 15;137(4):415-29 - PubMed
    1. Science. 1988 May 13;240(4854):889-95 - PubMed
    1. Science. 1988 Jun 24;240(4860):1759-64 - PubMed
    1. Cell. 1988 Oct 7;55(1):145-56 - PubMed

LinkOut - more resources