Comparison of the lateral flow immunoassays (LFIA) for the diagnosis of Helicobacter pylori infection

Abstract

Helicobacter pylori infection is the most common human infection where approximately 50% of the world populations are infected. The diagnosis of such infection is mainly done by endoscopy where gastric biopsies are examined for the presence of H. pylori. Such invasive approach is costly, time consuming and generally requires more than one test to confirm the infection. Serology on the other hand is a non-invasive approach that can detect H. pylori exposure. The lateral flow immunoassays (LFIA) support the serological approach and have the advantage of being fast, economic and require no additional equipment or experience. In this review the principles, components of the LFIA, sensitivities and specificities of the commercially available H. pylori test strips were compared and discussed.

Keywords: Colloidal gold; ELISA; Helicobacter pylori; ICA; Immunochromatography; LFIA; Lateral flow immunoassay; PCR; RUT; UBT; enzyme-linked immunosorbent assay; hCG; human chorionic gonadotropin.; immunochromatographic assay; lateral flow immunoassay; polymerase chain reaction; rapid urease test; urea breath test.