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. 2013 Oct;10(10):999-1002.
doi: 10.1038/nmeth.2634. Epub 2013 Sep 1.

Practical innovations for high-throughput amplicon sequencing

Derek S Lundberg  1 , Scott Yourstone, Piotr Mieczkowski, Corbin D Jones, Jeffery L Dangl
Affiliations

Practical innovations for high-throughput amplicon sequencing

Derek S Lundberg et al. Nat Methods. 2013 Oct.

Abstract

We describe improvements for sequencing 16S ribosomal RNA (rRNA) amplicons, a cornerstone technique in metagenomics. Through unique tagging of template molecules before PCR, amplicon sequences can be mapped to their original templates to correct amplification bias and sequencing error with software we provide. PCR clamps block amplification of contaminating sequences from a eukaryotic host, thereby substantially enriching microbial sequences without introducing bias.

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References

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