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. 2013 Nov;79(22):7110-3.
doi: 10.1128/AEM.01978-13. Epub 2013 Aug 30.

Cloning and heterologous expression of the thioviridamide biosynthesis gene cluster from Streptomyces olivoviridis

Masumi Izawa  1 , Takashi Kawasaki, Yoichi Hayakawa
Affiliations

Cloning and heterologous expression of the thioviridamide biosynthesis gene cluster from Streptomyces olivoviridis

Masumi Izawa et al. Appl Environ Microbiol. 2013 Nov.

Abstract

Thioviridamide is a unique peptide antibiotic containing five thioamide bonds from Streptomyces olivoviridis. Draft genome sequencing revealed a gene (the tvaA gene) encoding the thioviridamide precursor peptide. The thioviridamide biosynthesis gene cluster was identified by heterologous production of thioviridamide in Streptomyces lividans.

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Figures

Fig 1
Fig 1
Structure of thioviridamide (top) and amino acid sequence of TvaA (bottom).
Fig 2
Fig 2
Thioviridamide biosynthesis gene cluster from Streptomyces olivoviridis NA05001. (A) The tva gene cluster from Streptomyces olivoviridis NA05001 and four overlapping cosmids, pWE15TVAC1 to pWE15TVAC4. (B) Southern analysis of the EcoRI-digested cosmids.
Fig 3
Fig 3
HPLC analysis of the mycelial extract of S. lividans expressing the tva gene cluster. HPLC profiles of (a) standard thioviridamide and the culture extract of S. lividans carrying (b) a control plasmid (pWHM3) or (c) a tva gene cluster-containing plasmid (pWHM3-TVA).
See this image and copyright information in PMC

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