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. 2014 Jul-Aug;6(7-8):764-9.
doi: 10.1002/dta.1522. Epub 2013 Sep 2.

Determination of 4-bromo-2,5-dimethoxy-N-[(2-methoxyphenyl)methyl]-benzeneethanamine (25B-NBOMe) in serum and urine by high performance liquid chromatography with tandem mass spectrometry in a case of severe intoxication

Justin L Poklis  1 , Carol R Nanco, Michelle M Troendle, Carl E Wolf, Alphonse Poklis
Affiliations

Determination of 4-bromo-2,5-dimethoxy-N-[(2-methoxyphenyl)methyl]-benzeneethanamine (25B-NBOMe) in serum and urine by high performance liquid chromatography with tandem mass spectrometry in a case of severe intoxication

Justin L Poklis et al. Drug Test Anal. 2014 Jul-Aug.

Abstract

We present a case of 4-bromo-2,5-dimethoxy-N-[(2-methoxyphenyl)methyl]-benzeneethanamine (25B-NBOMe), an N-benzyl phenethylamines derivative, intoxication and a high performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) method for detection and quantification of 25B-NBOMe. A 19-year-old male was found unresponsive with generalized grand mal seizure activity. On the second day of hospitalization, a friend admitted that the patient used 'some unknown drug' called 25B. Serum and urine collected were sent to the Virginia Commonwealth University Medical Center Toxicology Laboratory for analysis. An HPLC-MS/MS method for the identification and quantification of 25B-NBOMe using 2-(2,5-dimethoxyphenyl)-N-(2-methoxybenzyl)ethanamine (25H-NBOMe) as the internal standard (ISTD) was developed. As this is a novel, single-case presentation, an assay validation was performed prior to testing to ensure the reliability of the analytical results. The serum and urine specimens were determined to contain 180 pg/ml and1900 pg/ml of 25B-NBOMe, respectively.

Keywords: 25B-MBOMe; HPLC-MS/MS; bath salts; designer drugs; overdose.

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Figures

Figure 1
Figure 1
Chemical Structure of 25B-NBOMe and 25H-NBOMe.
Figure 2
Figure 2
Chromatographic separation of 25B-NBOMe and 25H-NBOMe in patient serum sample.
See this image and copyright information in PMC

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