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. 2013:1012:135-44.
doi: 10.1007/978-1-62703-429-6_10.

Methods to quantify microRNAs in the Myc gene network for posttranscriptional gene repression

Rui Song  1 , Nicole Sponer, Lin He
Affiliations

Methods to quantify microRNAs in the Myc gene network for posttranscriptional gene repression

Rui Song et al. Methods Mol Biol. 2013.

Abstract

As a global transcription factor, Myc regulates both protein-coding genes and noncoding microRNA genes. Myc-activated or repressed miRNAs are involved in various pathways to affect tumorigenesis, mediate apoptosis, proliferation, angiogenesis, metastasis, and metabolism downstream of Myc. Functional characterization of miRNAs in the Myc network requires the accurate detection and quantification of miRNA expression levels. Here, we describe two widely used methodologies to determine miRNA expression, including miRNA real-time PCR and miRNA northern analysis.

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Figures

Fig. 1
Fig. 1
Representatives Myc-activated or repressed miRNAs in various cellular processes during tumor development, including angiogenesis, cell proliferation, metastasis, apoptosis and metabolism
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