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. 2013:529:1-21.
doi: 10.1016/B978-0-12-418687-3.00001-X.

Explanatory chapter: PCR primer design

Rubén Álvarez-Fernández  1
Affiliations

Explanatory chapter: PCR primer design

Rubén Álvarez-Fernández. Methods Enzymol. 2013.

Abstract

This chapter is intended as a guide on polymerase chain reaction (PCR) primer design (for information on PCR, see General PCR and Explanatory Chapter: Troubleshooting PCR). In the next section, general guidelines will be provided, followed by a discussion on primer design for specific applications. A list of recommended software tools is shown at the end.

Keywords: Homologous recombination; Multiplex PCR; PCR Primer design; Primer length; Real-time PCR; Reverse-transcription PCR (RT-PCR); Target cloning.

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