doi: 10.1016/B978-0-12-418687-3.00007-0.
Molecular cloning
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- PMID: 24011038
- DOI: 10.1016/B978-0-12-418687-3.00007-0
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Molecular cloning
Methods Enzymol.
2013.
Abstract
This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
Keywords: E. coli; Ligation; Molecular cloning; Primer design; Successful ligation events.
Copyright © 2013 Elsevier Inc. All rights reserved.
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