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. 2013 Jul;7(2):125-9.
doi: 10.4103/0973-6247.115570.

Seroprevalence of human immunodeficiency virus in north Indian blood donors using third and fourth generation Enzyme linked immunosorbent assay

Sheetal Malhotra  1 , Neelam Marwaha, Karan Saluja
Affiliations

Seroprevalence of human immunodeficiency virus in north Indian blood donors using third and fourth generation Enzyme linked immunosorbent assay

Sheetal Malhotra et al. Asian J Transfus Sci. 2013 Jul.

Abstract

Background: The percentage of HIV cases attributable to blood transfusion has decreased significantly in the last decade. The newer 4(th) generation Enzyme linked immunosorbent assay (ELISA) has been shown to have increased sensitivity compared to 3(rd) generation ELISA.

Objectives: To estimate the seroprevalence of HIV among blood donors using 4(th) generation ELISA assay and to compare it with the 3(rd) generation ELISA.

Materials and methods: This prospective study involved 10,200 blood donors- 6,800 were voluntary donors (3400-students and 3400-non students) and 3400 were replacement donors. All blood units were tested with 3(rd) as well as 4(th) generation ELISA. All samples found reactive or in grey zone with either 3(rd) or 4(th) generation ELISA were retested by Western blot (WB).

Results: The seroprevalence of HIV was estimated to be 1.37/1000 donations (0.14%) with 3(rd) generation ELISA compared to 3.62/1000 donations (0.36%) with 4(th) generation ELISA (p>0.05). The seroprevalence of HIV among voluntary donors was estimated to be 1.32/1000 donations (0.13%) with 3(rd) generation ELISA and 3.67/1000 donations (0.36%) with 4(th) generation ELISA. The prevalence of HIV among replacement donors was 1.47/1000 donations (0.15%) with 3(rd) generation ELISA and 3.52/1000 donations (0.35%) with 4(th) generation ELISA.

Conclusion: 4(th) generation HIV ELISA detects a higher number of seroreactive donors compared to 3(rd) generation ELISA. However, larger studies are required with confirmatory tests for both 3(rd) and 4(th) generation ELISA for making any policy changes.

Keywords: Enzyme linked immunosorbent assay; Human immunodeficiency virus; blood donors; transfusion associated HIV/AIDS; window period.

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Conflict of interest statement

Conflict of Interest: None declared.

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References

    1. Mollison PL, Engelfriet CP, Contreras M. Infectious agents transmitted by transfusion. In: Klein HG, Anstee DJ, editors. Mollison's Blood Transfusion in Clinical Medicine. 11th ed. Oxford: Blackwell; 2005. pp. 701–73.
    1. National AIDS Control Organization. Annual Report NACO 20010-11. [Online] [Last cited on 2012 Jan 12]. Available from: URL:http://www.nacoonline.org .
    1. Weber B. Screening of HIV infection: Role of molecular and immunological assays. Expert Rev Mol Diagn. 2006;6:399–411. - PubMed
    1. Sharma RR, Cheema R, Vajpayee M, Rao U, Kumar S, Marwaha N, et al. Prevalence of markers of transfusion transmissible diseases in voluntary and replacement blood donors. Natl Med J Ind. 2004;17:19–21. - PubMed
    1. Mallik V. 16th ed. Lucknow: Eastern Book Company; 2003. Laws related to Drugs and Cosmetics Act 1940; pp. 279–303.