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Comment
. 2013 Sep 3:2:e01387.
doi: 10.7554/eLife.01387.

Making the most of methylation

Michiel Vermeulen  1
Affiliations
Comment

Making the most of methylation

Michiel Vermeulen. Elife. .

Abstract

A high-throughput screening approach based on a protein microarray reveals that many human transcription factors interact specifically with methylated promoter sequences.

Keywords: DNA methylation; Human; epigenetics; protein microarray; protein-DNA interactions; transcription factors; transcription regulation.

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Conflict of interest statement

Competing interests:The author declares that no competing interests exist.

Figures

Figure 1.
Figure 1.
Some human transcription factors can bind to both methylated and non-methylated DNA sequences. Hu et al. examined the ability of 17 human transcription factors to bind to 150 different DNA motifs containing methylated or non-methylated CpG islands. Each row represents one transcription factor. For each motif, some transcription factors bound only to the methylated version (red), some to only the non-methylated version (blue); some to both methylated and non-methylated versions (green), and some to neither (grey). From Figure 2a in Hu et al., 2013.
See this image and copyright information in PMC

Comment on

References

    1. Bartels SJ, Spruijt CG, Brinkman AB, Jansen PW, Vermeulen M, Stunnenberg HG. 2011. A SILAC-based screen for Methyl-CpG binding proteins identifies RBP-J as a DNA methylation and sequence-specific binding protein. PLOS ONE 6:e25884. 10.1371/journal.pone.0025884 - DOI - PMC - PubMed
    1. Bartke T, Vermeulen M, Xhemalce B, Robson SC, Mann M, Kouzarides T. 2010. Nucleosome-interacting proteins regulated by DNA and Histone methylation. Cell 143:470–84. 10.1016/j.cell.2010.10.012 - DOI - PMC - PubMed
    1. Baubec T, Ivanek R, Lienert F, Schubeler D. 2013. Methylation-dependent and -independent genomic targeting principles of the MBD protein family. Cell 153:480–92. 10.1016/j.cell.2013.03.011 - DOI - PubMed
    1. Bogdanovic O, Long SW, van Heeringen SJ, Brinkman AB, Gomez-Skarmeta JL, Stunnenberg HG, et al. 2011. Temporal uncoupling of the DNA methylome and transcriptional repression during embryogenesis. Genome Res 21:1313–27. 10.1101/gr.114843.110 - DOI - PMC - PubMed
    1. Defossez PA, Stancheva I. 2011. Biological functions of methyl-CpG-binding proteins. Prog Mol Biol Transi Sci 101:377–98. 10.1016/B978-0-12-387685-0.00012-3 - DOI - PubMed

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