Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Apr;19(2):188-95.
doi: 10.4103/0971-6866.116124.

CD14 C-159T polymorphism and its association with chronic lung diseases: A pilot study on isocyanate exposed population of Central India

Protiti Bose  1 , Rashmi Bathri, Sajal De, K K Maudar
Affiliations

CD14 C-159T polymorphism and its association with chronic lung diseases: A pilot study on isocyanate exposed population of Central India

Protiti Bose et al. Indian J Hum Genet. 2013 Apr.

Abstract

Context: CD14 functions as a multifunctional receptor for bacterial cell wall components including endotoxin and lipopolysaccharide and is likely to influence the cytokine profile and subsequent immunoglobulin E production in response to antigen/allergen contact in allergic phenotypes.

Aims: The present study was to investigate genetic polymorphism in CD14 gene - 159C/T, which may be one of the risk factor for increased prevalence of Chronic Lung Diseases in the Central India.

Settings and design: Survivors of Methyl isocyanates toxicity in Bhopal still suffering from various respiratory ailments were examined.

Materials and methods: Polymerase chain reaction-restriction fragment length polymorphism was performed to determine the polymorphism of C-159T.

Results: The genotype and allelic frequencies were in Hardy-Weinberg's equilibrium. Prevalence of CC, CT, and TT were 5.5%, 22.2% and 9.25% respectively in asthmatics; 16.6%, 20.3% and 5.5% respectively in chronic obstructive pulmonary disease (COPD) patients and 5.5%, 14.8% and 1.85 respectively among interstitial lung disorder (ILD) patients; whereas the control cohort with no methyl isocyanate exposure displayed (CC, CT, and TT) cytosine, thymine as 2%, 1.6% and 2% respectively. Increased risk of Asthma among those carrying TT genotype and T allele (odds ratio [OR] =2.61 and 2.02 respectively).

Conclusion: COPD risk significantly found among those with CC genotype and C allele (OR = 2.81 and 1.50 respectively), whereas ILD risk found significantly among CT genotype and C allele (OR = 1.75 and 1.40 respectively). Therefore, single nucleotide polymorphism (SNP) C-159T polymorphism in CD14 gene might be a risk factor for development of CLD in this population.

Keywords: Asthma; Bhopal; CD14; chronic obstructive methyl isocyanate; pulmonary disease.

PubMed Disclaimer

Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Regression analysis of total serum IgE level in isocyanate exposed patients and their radial distance of residence from UCIL plant
Figure 2
Figure 2
Microsatellite instability displayed using (CA)8RG microsatellite primer among methyl isocyanate gas victims suffering from chronic obstructive pulmonary disease, asthma and interstitial lung disorder (lane 2, 4 - 6 respectively) as compared with non - gas exposed with normal lung functioning (lane 3c1) and gas victims with normal lung functioning (lane 3c2) ; lane 1 is 100 bp marker. Arrows showing altered bands
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Bhargava A, Punde RP, Pathak N, Dabadghao S, Desikan P, Jain A, et al. Status of inflammatory biomarkers in the population that survived the Bhopal gas tragedy: A study after two decades. Ind Health. 2010;48:204–8. - PubMed
    1. Vijayan VK. Toxic trauma affecting the lungs with special reference to the Bhopal disaster. J Res Sci. 2006;8:105–111.
    1. Mishra PK, Samarth RM, Pathak N, Jain SK, Banerjee S, Maudar KK. Bhopal Gas Tragedy: Review of clinical and experimental findings after 25 years. Int J Occup Med Environ Health. 2009;22:193–202. - PubMed
    1. De S. Retrospective analysis of lung function abnormalities of Bhopal gas tragedy affected population. Indian J Med Res. 2012;135:193–200. - PMC - PubMed
    1. Vijayan VK, Sankaran K. Relationship between lung inflammation, changes in lung function and severity of exposure in victims of the Bhopal tragedy. Eur Respir J. 1996;9:1977–82. - PubMed