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. 2013 Sep 3;8(9):e74738.
doi: 10.1371/journal.pone.0074738. eCollection 2013.

Expression of SIRT1 and DBC1 is associated with poor prognosis of soft tissue sarcomas

Jung Ryul Kim  1 , Young Jae Moon, Keun Sang Kwon, Jun Sang Bae, Sajeev Wagle, Taek Kyun Yu, Kyoung Min Kim, Ho Sung Park, Ju-Hyung Lee, Woo Sung Moon, Ho Lee, Myoung Ja Chung, Kyu Yun Jang
Affiliations

Expression of SIRT1 and DBC1 is associated with poor prognosis of soft tissue sarcomas

Jung Ryul Kim et al. PLoS One. .

Abstract

Recently, the roles of SIRT1 and deleted in breast cancer 1 (DBC1) in human cancer have been extensively studied and it has been demonstrated that they are involved in many human carcinomas. However, their clinical significance for soft-tissue sarcomas has not been examined. In this study, we evaluated the expression and prognostic significance of the expression of SIRT1, DBC1, P53, β-catenin, cyclin D1, and KI67 in 104 cases of soft-tissue sarcomas.

Results: Immunohistochemical expression of SIRT1, DBC1, P53, β-catenin, and cyclin D1 were seen in 71%, 74%, 53%, 48%, and 73% of sarcomas, respectively. The expression of SIRT1, DBC1, P53, β-catenin, and cyclin D1 were significantly correlated with advanced clinicopathological parameters such as higher clinical stage, higher histological grade, increased mitotic counts, and distant metastasis. The expression of SIRT1, DBC1, P53, β-catenin, cyclin D1, and KI67 were significantly correlated with each other and positive expression of all of these predicted shorter overall survival and event-free survival by univariate analysis. Multivariate analysis revealed the expression of SIRT1 as an independent prognostic indicator for overall survival and event-free survival of sarcoma patients. In conclusion, this study demonstrates that SIRT1- and DBC1-related pathways may be involved in the progression of soft-tissue sarcomas and can be used as clinically significant prognostic indicators for sarcoma patients. Moreover, the SIRT1- and DBC1-related pathways could be new therapeutic targets for the treatment of sarcomas.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Immunohistochemical expression of SIRT1, DBC1, β-catenin, cyclin D1, P53, and Ki67 in various soft tissue sarcomas.
All markers are expressed primarily in the nuclei of the tumor cells. Abbreviations: FS, adult fibrosarcoma; LMS, leiomyosarcoma; US, undifferentiated sarcoma; SS, synovial sarcoma; ES, Ewing sarcoma; LS, liposarcoma; RMS, rhabdomyosarcoma; MPNST, malignant peripheral nerve sheath tumor; AS, angiosarcoma. Original magnification, x400.
Figure 2
Figure 2. Kaplan-Meier survival analysis of soft tissue sarcoma patients.
Overall survival and event-free survival according to tumor stage (A), histological grade (B), and the expression of SIRT1 (C), DBC1 (D), P53 (E), β-catenin (F), cyclin D1 (G), and Ki67 (H).
See this image and copyright information in PMC

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