Non contiguous-finished genome sequence and description of Peptoniphilus senegalensis sp. nov

Abstract

Peptoniphilus senegalensis strain JC140(T) sp. nov., is the type strain of P. senegalensis sp. nov., a new species within the genus Peptoniphilus. This strain, whose genome is described here, was isolated from the fecal flora of a healthy patient. P. senegalensis strain JC140(T) is an obligate Gram-positive anaerobic coccus. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 1,840,641 bp long genome (1 chromosome but no plasmid) exhibits a G+C content of 32.2% and contains 1,744 protein-coding and 23 RNA genes, including 3 rRNA genes.

Keywords: Peptoniphilus senegalensis; genome.

Figure 1

Phylogenetic tree highlighting the position of Peptoniphilus senegalensis strain JC140^T relative to other type strains within the Peptoniphilus genus. GenBank accession numbers are indicated in parentheses. Sequences were aligned using CLUSTALW, and phylogenetic inferences obtained using the maximum-likelihood method within the MEGA software. Numbers at the nodes are percentages of bootstrap values obtained by repeating the analysis 500 times to generate a majority consensus tree. Anaerococcus prevotii was used as outgroup. The scale bar represents a 2% nucleotide sequence divergence.

Figure 2

Gram staining of P. senegalensis strain JC140^T

Figure 3

Transmission electron microscopy of P. senegalensis strain JC140^T, using a Morgani 268D (Philips) at an operating voltage of 60kV.The scale bar represents 200 nm.

Figure 4

Reference mass spectrum from P. senegalensis strain JC140^T. Spectra from 12 individual colonies were compared and a reference spectrum was generated.

Figure 5

Graphical circular map of the chromosome. From the outside to the inside: genes on the forward strand (colored by COG categories), genes on the reverse strand (colored by COG categories), RNA genes (tRNAs green, rRNAs red), G+C content and GC skew.

Figure 6

Gene distribution in COG functional categories in P. senegalensis (orange), P. harei (blue), P. timonensis (red) and P. indolicus (green) chromosomes.