Investigation of the direct hepatic effects of intramuscular interleukin-8 injection in an experimental rabbit model

Abstract

Background: The aim of this study was to investigate the effects of intramuscular IL-8 injection on hepatic tissues using an in vivo histopathological animal model.

Material and methods: Twelve New Zealand white rabbits were used for this randomized, controlled, single-blinded interventional study. For 6 days, 1 gluteus maximus muscle was injected daily with 1 mcg/kg of IL-8 in 6 rabbits (Group A). The remaining 6 rabbits (to determine to normal porto-hepatic morphology of the rabbit genus) were in the sham group (Group B). At the end of the 7th day, all rabbits were killed and livers were meticulously harvested. Microscopically, regional tissues were scored according to portal inflammation, focal necrosis, piecemeal necrosis, and total impact.

Results: Total impact score, portal inflammation, focal necrosis, and piecemeal necrosis were the histopathologic changes present in a higher incidence in the IL-8 group compared with the control group. The differences were significant when the groups were compared according to total impact score, portal inflammation, focal necrosis, and piecemeal necrosis according to Pearson's correlation (p<0.05). The most significant differences were detected at the total impact scores (p=0.002) and the portal inflammation scores (p=0.008).

Conclusions: Our results showed that IL-8 may damage hepatocytes. This can be the determined target for new therapeutic strategies. Further trials should be designed to obtain definitive results.

Figure 1

(A) Native portal area [no lymphocytic infiltration] (×200, H&E). (B) Hepatic tissue with focal necrosis (×200, H&E). (C) Moderate lymphocytic infiltration in portal area (×100, H&E). (D) Intensive lymphocytic infiltration in portal area (×100, H&E). (E) Severe Piecemeal necrosis (×100, H&E).