Molecular cloning and expression of four actin isoforms during Artemia development
- PMID: 2402445
- PMCID: PMC332145
- DOI: 10.1093/nar/18.17.5219
Molecular cloning and expression of four actin isoforms during Artemia development
Abstract
Four cDNA clones coding for different Artemia actin isoforms have been isolated. Three of the clones contain the complete coding sequences while the fourth one lacks 145 bases, coding for the 49 amino terminal amino acids of the protein. The amino acid sequences predicted for the four actin isoforms identified are highly homologous to insect actins as well as to vertebrate cytoplasmic actins. The four identified cDNA clones code for mRNAs of 5.2, 1.9, 1.6 and 1.8 kb, respectively, whose expression is regulated during development. Three of the actin mRNAs are present in cryptobiotic embryos while the other is not. The steady-state levels of all four mRNAs increase during development to reach maximal levels by 10-15 hours of development and decrease thereafter. The total number of actin genes encoded in the Artemia genome has been estimated as 8 to 10 by Southern analysis of total DNA.
Similar articles
-
Expression of actin isoforms in Artemia.Symp Soc Exp Biol. 1992;46:131-7. Symp Soc Exp Biol. 1992. PMID: 1341031
-
Complementary DNA cloning of a protein highly homologous to mammalian sarcoplasmic reticulum Ca-ATPase from the crustacean Artemia.J Mol Biol. 1989 Dec 20;210(4):737-48. doi: 10.1016/0022-2836(89)90106-x. J Mol Biol. 1989. PMID: 2533270
-
Cloning of a cDNA encoding an Artemia franciscana Na/K ATPase alpha-subunit.Gene. 1991 Sep 15;105(2):197-204. doi: 10.1016/0378-1119(91)90151-z. Gene. 1991. PMID: 1657719
-
Isolation and characterization of the gene coding for Artemia franciscana TATA-binding protein: expression in cryptobiotic and developing embryos.Biochim Biophys Acta. 1999 Jun 9;1445(3):271-82. doi: 10.1016/s0167-4781(99)00052-4. Biochim Biophys Acta. 1999. PMID: 10366711
-
Divergence and differential expression of actin gene families in higher plants.Int Rev Cytol. 1991;125:139-63. doi: 10.1016/s0074-7696(08)61218-8. Int Rev Cytol. 1991. PMID: 2032783 Review. No abstract available.
Cited by
-
A TaqI RFLP for the human arylsulfatase A gene.Nucleic Acids Res. 1990 Nov 25;18(22):6746. doi: 10.1093/nar/18.22.6746-a. Nucleic Acids Res. 1990. PMID: 1979166 Free PMC article.
-
Actin gene structure in two Artemia species, A. franciscana and A. parthenogenetica.J Mol Evol. 1996 Sep;43(3):224-35. doi: 10.1007/BF02338830. J Mol Evol. 1996. PMID: 8703088
-
Insect muscle actins differ distinctly from invertebrate and vertebrate cytoplasmic actins.J Mol Evol. 1992 May;34(5):406-15. doi: 10.1007/BF00162997. J Mol Evol. 1992. PMID: 1602494
-
Actin genes and their expression in pacific white shrimp, Litopenaeus vannamei.Mol Genet Genomics. 2018 Apr;293(2):479-493. doi: 10.1007/s00438-017-1397-y. Epub 2017 Nov 30. Mol Genet Genomics. 2018. PMID: 29189957
-
New nucleotide sequence data on the EMBL File Server.Nucleic Acids Res. 1990 Nov 25;18(22):6747-51. doi: 10.1093/nar/18.22.6747. Nucleic Acids Res. 1990. PMID: 2251162 Free PMC article. No abstract available.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
