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. 2013 Dec;51(12):4193-5.
doi: 10.1128/JCM.01876-13. Epub 2013 Sep 11.

Evaluation of pneumococcal serotyping by multiplex PCR and quellung reactions

Sandra S Richter  1 , Kristopher P Heilmann, Cassie L Dohrn, Fathollah Riahi, Daniel J Diekema, Gary V Doern
Affiliations

Evaluation of pneumococcal serotyping by multiplex PCR and quellung reactions

Sandra S Richter et al. J Clin Microbiol. 2013 Dec.

Abstract

Screening of 1,750 pneumococcal isolates for common serotypes by PCR was followed by Quellung reaction analysis of PCR-negative isolates with a comparison to the conventional (Quellung reaction only) approach. PCR agreed with Quellung reaction results for 99% of isolates. The sequential PCR/Quellung reaction algorithm is accurate and more cost-effective than the conventional approach.

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Figures

Fig 1
Fig 1
The leftmost five lanes show band sizes expected for (from left to right) serotype 6A/B/C/D (250 bp), 3 (371 bp), 19A (478 bp), 22F/22A (643 bp), and 7F (826 bp) isolates (6). The rightmost lane is a standard ladder, and the values shown to the right are in bp. The band at the bottom of each lane represents unused primers.
See this image and copyright information in PMC

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