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. 2008 Jan;39(1):143-50.
doi: 10.1590/S1517-838220080001000029. Epub 2008 Mar 1.

Biodiversity and phylogenetic analysis of culturable bacteria indigenous to Khewra salt mine of pakistan and their industrial importance

Nasrin Akhtar  1 , Muhammad A Ghauri, Aamira Iqbal, Munir A Anwar, Kalsoom Akhtar
Affiliations

Biodiversity and phylogenetic analysis of culturable bacteria indigenous to Khewra salt mine of pakistan and their industrial importance

Nasrin Akhtar et al. Braz J Microbiol. 2008 Jan.

Abstract

Culturable bacterial biodiversity and industrial importance of the isolates indigenous to Khewra salt mine, Pakistan was assessed. PCR Amplification of 16S rDNA of isolates was carried out by using universal primers FD1 and rP1and products were sequenced commercially. These gene sequences were compared with other gene sequences in the GenBank databases to find the closely related sequences. The alignment of these sequences with sequences available from GenBank database was carried out to construct a phylogenetic tree for these bacteria. These genes were deposited to GenBank and accession numbers were obtained. Most of the isolates belonged to different species of genus Bacillus, sharing 92-99% 16S rDNA identity with the respective type strain. Other isolates had close similarities with Escherichia coli, Staphylococcus arlettae and Staphylococcus gallinarum with 97%, 98% and 99% 16S rDNA similarity respectively. The abilities of isolates to produce industrial enzymes (amylase, carboxymethylcellulase, xylanase, cellulase and protease) were checked. All isolates were tested against starch, carboxymethylcellulose (CMC), xylane, cellulose, and casein degradation in plate assays. BPT-5, 11,18,19 and 25 indicated the production of copious amounts of carbohydrates and protein degrading enzymes. Based on this study it can be concluded that Khewra salt mine is populated with diverse bacterial groups, which are potential source of industrial enzymes for commercial applications.

Keywords: Biodiversity; Highly saline environments; Industrially important enzymes; PCR amplification of 16S rDNA; characterization.

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Figures

Figure 1
Figure 1
The inferred relationship, based on partial 16S rDNA sequence of the Khewra salt mine isolates, isolated in this study (in bold) to other bacteria. The tree was rooted with Escherichia coli. Scale bar represents the number of inferred nucleotide substitution per site. Bootstrap values (100 replicates) are shown at the nodes.
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