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. 2008 Jul;39(3):489-93.
doi: 10.1590/S1517-838220080003000014. Epub 2008 Sep 1.

In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil

Daniel M Aguiar  1 , Mitika K Hagiwara, Marcelo B Labruna
Affiliations

In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil

Daniel M Aguiar et al. Braz J Microbiol. 2008 Jul.

Abstract

An Ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in São Paulo Municipality, state of São Paulo, Brazil. The isolate was characterized by PCR and DNA sequencing of portions of the ehrlichial genes dsb, 16SrRNA, and p28. Partial dsb and 16S rRNA sequences were identical to three and five other E. canis strains, respectively, from different countries and continents (including North America, Africa, Asia and Europe). Conversely, the p28 partial sequence for this E. canis (São Paulo) differed by 1, 2, and 2 nucleotides from the corresponding sequences of the E. canis strains Jake (from USA), Oklahoma (USA), and VHE (Venezuela), respectively. The results in this study indicate that E. canis is the only recognized Ehrlichia species infecting dogs in Brazil.

Keywords: Dog; Ehrlichia canis; Ehrlichiosis; Molecular Characterization.

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Figures

Figure 1
Figure 1
Ehrlichia morulae (São Paulo strain) within DH82 cells stained by Diff-Quik staining (original magnification 100x).
See this image and copyright information in PMC

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