Xylanase production with xylan rich lignocellulosic wastes by a local soil isolate of Trichoderma viride

Abstract

In the present study, cultural and nutritional conditions for enhanced production of xylanase by a local soil isolate of Trichoderma viride, using various lignocellulosic substrates in submerged culture fermentation have been optimized. Of the lignocellulosics used, maize straw was the best inducer followed by jowar straw for xylanase production. The highest activity achieved was between 14 to 17 days of fermentation. A continuous increase in xylanase production was observed with increasing level of lignocellulosics in the medium and highest activity was observed with maize straw at 5% level. Xylanase production with higher levels of lignocellulosics (3 to 5%) of maize, jowar and barseem was found to be higher as compared to that with commercial xylan as carbon source. Sodium nitrate was the best nitrogen source among the six sources used. Maximum xylanase production was achieved with initial medium pH of 3.5-4.0 and incubation temperature of 25ºC.The enzyme preparation was effective in bringing about saccharification of different lignocellulosics. The xylanase production could be further improved by using alkali treated straw as carbon source.

Keywords: Lignocellulosics; Submerged fermentation; Trichoderma viride; Xylanase production.

Figure 1

Effect of incubation temperature (Fig. 1 a) and pH (Fig. 1b) on xylanase production by T. viride (S34) at 25ºC using 1% maize straw as carbon source.

Figure 2

Saccharification of different lignocellulosic substrates with fibrolytic enzymes at 0 (control ♦), 0.5 ml g-¹ (▲) or 1 ml g-¹ (■)