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. 2009 Apr;40(2):314-20.
doi: 10.1590/S1517-838220090002000019. Epub 2009 Jun 1.

Characterization of Mycobacterium tuberculosis complex isolated from iranian and afghani patients by spoligotyping method

Rashid Ramazanzadeh  1 , Parisa Farnia, Nour Amirmozafari
Affiliations

Characterization of Mycobacterium tuberculosis complex isolated from iranian and afghani patients by spoligotyping method

Rashid Ramazanzadeh et al. Braz J Microbiol. 2009 Apr.

Abstract

Designing newer drugs, vaccines, and diagnostic techniques is dependent on better understanding of M. tuberculosis virulence mechanism. In this study the prevalence of pcaA gene was determined in M. tuberculosis strains typed by spoligotyping. The associated risk factors among patients with different nationalities residing in Iran were also determined. The isolated M. tuberculosis strains have been characterized by performing susceptibility tests against four first-line antituberculosis drugs and were then subjected to spoligotyping characterization. PCR was used for detection of pcaA gene and its nucleotide sequence was also determined. Spoligotyping of M. tuberculosis strains resulted in 140 different patterns. One hundred twenty two (87.1%) of these spoligotype isolates were unique and reported for the first time. The remaining18 (12.8%) spoligotype patterns were previously reported from other geographical regions of the world. Haarlem family was most prevalent than other genotype. Antibiotic resistances were higher in those isolated from the Iranian patients. The pcaA gene was detected in M. tuberculosis clinical isolates but not in saprophyte strains such as M. kansasi. The results showed that, spread of M. tuberculosis strains belonging to the Beijing family among Iranian patients has to be considered seriously. This study confirmed the widespread existence of pcaA gene in almost all the clinical isolates. It is also important to undertake studies to identify which factors are the most significant to consider in tuberculosis control program.

Keywords: drugs; pcaA; resistance; spoligotyping; tuberculosis.

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Figures

Figure 1
Figure 1
Resistance patterns to antituberculosis drugs among M. tuberculosis isolates from 523 TB patients (P<0.002).
Figure 2
Figure 2
The distribution pattern of Multidrug-Resistance (MDR) strains in reference to patient’s nationalities (P<0.005).
Figure 3
Figure 3
Gel electrophoresis of the PCR amplified pcaA gene products (M. tuberculosis lanes 1, 2, 3, 5, 6, 7, 8, 9, and 11; M. Kansasi lane 4; Negative control lane 10, lane 12 marker 50–1000bp).
See this image and copyright information in PMC

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