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. 2009 Apr;40(2):373-9.
doi: 10.1590/S1517-838220090002000030. Epub 2009 Jun 1.

Evaluation of a lipopeptide biosurfactant from Bacillus natto TK-1 as a potential source of anti-adhesive, antimicrobial and antitumor activities

Xiao-Hong Cao  1 , Zhen-Yu Liao, Chun-Ling Wang, Wen-Yan Yang, Mei-Fang Lu
Affiliations

Evaluation of a lipopeptide biosurfactant from Bacillus natto TK-1 as a potential source of anti-adhesive, antimicrobial and antitumor activities

Xiao-Hong Cao et al. Braz J Microbiol. 2009 Apr.

Abstract

A lipopeptide biosurfactant produced by Bacillus natto TK-1 has a strong surface activity. The biosurfactant was found to be an anti-adhesive agent against several bacterial strains, and also showed a broad spectrum of antimicrobial activity. The biosurfactant induced a significant reduction in tumor cells viability in a dose-dependent manner.

Keywords: Biosurfactant; anti-adhesive; antimicrobial activity; antitumor.

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Figures

Figure 1
Figure 1
(a) Surface tensions of biosurfactant at various concentrations. Surface tension was measured by the Ring method at 20°C; (b) Relationship between diameter of oil displacement and various concentrations of biosurfactant. Each sample was repeated in triplicate and the control plate was evaluated with PBS.
Figure 2
Figure 2
Antimicrobial activity of biosurfactant at various concentrations (0-3.2 g/L) against B. cinerea.
Figure 3
Figure 3
Effects of biosurfactant on the viability of tumor and normal cells. K562 and BEL-7402 cells (a), and BRL and HEK293 (b) were treated with biosurfactant at the indicated concentrations for 48 h. The viability was determined by MTT assay. Each value represents mean±S.D. of three independent experiments.
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