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. 2009 Jul;40(3):522-34.
doi: 10.1590/S1517-838220090003000014. Epub 2009 Sep 1.

Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.)

José Edson Fontes Figueiredo  1 , Eliane Aparecida Gomes, Claudia Teixeira Guimarães, Ubiraci Gomes de Paula Lana, Marta Aparecida Teixeira, Guilherme Vitor Corrêa Lima, Wellington Bressan
Affiliations

Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.)

José Edson Fontes Figueiredo et al. Braz J Microbiol. 2009 Jul.

Abstract

Endophytic bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of whole-cell protein extract of forty-two isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosomal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains. Among the forty-two isolates identified by rDNA sequencing, Bacillus subitilis and B. pumilus were the most frequenty species (15 and 12 isolates, respectively) followed by B. licheniformes (7 isolates), B. cereus (5 isolates) and B. amiloliquefascens (3 isolates). According to present results, SDS-PAGE technique could be used as a fast and cheap first tool for identifying inter-specific variation in maize endophytic bacterial collections while rDNA sequencing could be applied for analyzing intra-specific variation among isolates with similar protein profile as well as for taxonomic studies.

Keywords: Bacillus; SDS-PAGE; endophytic bacteria; rDNA sequencing; sweet corn.

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Figures

Figure 1
Figure 1
Electrophoretic profile (SDS-PAGE) of whole protein extract of forty-two endophytic bacteria isolated from tropical sweet corn. Numbers 1 to 42 indicate bacterial isolates 1 to 42, respectively. M = Protein Molecular weight markers (Rainbow, New England Biolabs, Ipswich, MA).
See this image and copyright information in PMC

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