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. 2009 Oct;40(4):747-56.
doi: 10.1590/S1517-83822009000400004. Epub 2009 Dec 1.

Screening of microbial lipases and evalutaion of their potential to produce glycerides with high gamma linolenic acid concentration

Patricia B L Fregolente  1 , Leonardo V Fregolente, Maria R W Maciel, Patricia O Carvalho
Affiliations

Screening of microbial lipases and evalutaion of their potential to produce glycerides with high gamma linolenic acid concentration

Patricia B L Fregolente et al. Braz J Microbiol. 2009 Oct.

Abstract

Gamma linolenic acid (GLA, 18:3, cis- 6,9,12- octadecatrienoic acid), an important compound in n- 6 eicosanoid family biosynthesis, occurs in the lipids of a few plant and microbial sources. This study focused on the screening of microbial strains with suitable lipase activity for enrichment of GLA by selective hydrolysis of the borage oil (21.6 % of GLA/total fatty acids). Firstly, 352 microrganisms were tested for their lipolytic capacity using screening techniques on agar plates containing borage oil, strains were then selected and screened for their activity (U/mg) using both submerged fermentation (SmF) and solid state fermentation (SSF). The rate of hydrolysis and the selective preference of these hydrolytic enzymes towards fatty acids, with a special focus on enrichment of GLA were studied and compared with those obtained by two commercially-available lipases. Only one of the lipases tested during this study displayed selectivity, discriminating the GLA during the hydrolysis reaction. Using the enzymatic extract from Geotrichum candidum as a biocatalyst of the reaction, it was possible to obtain a percentage of 41.7% of GLA in acylglycerols fraction when the borage oil was treated in a fixed-bed reactor for 24 hours at 30ºC.

Keywords: Geotrichum candidum; borage oil; gamma linolenic acid; lipase.

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Figures

Figure 1
Figure 1
Content of the acylglycerols (TG, DG and MG) and FFA, derived by hydrolysis of borage oil with selected lipases. 1. A. niger, 2. F oxysporum, 3. G. candidum, 4. P. solitum, 5. R. javanicus, 6. C. cylindracea (Sigma), 7. A. niger (Aldrich). Reaction conditions are described in Materials and Methods
Figure 2
Figure 2
Change in GLA content in the acylglycerols (TG, DG and MG) and FFA fractions during the course of hydrolysis of borage oil, catalyzed by native G. candidum lipase produced in two different growing media. Reaction conditions are described in Materials and Methods
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