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. 2010 Apr;41(2):270-7.
doi: 10.1590/S1517-83822010000200001. Epub 2010 Jun 1.

Antimycobacterial susceptibility testing methods for natural products research

Juan Gabriel Bueno Sánchez  1 , Vladimir V Kouznetsov
Affiliations

Antimycobacterial susceptibility testing methods for natural products research

Juan Gabriel Bueno Sánchez et al. Braz J Microbiol. 2010 Apr.

Abstract

The emergence of multidrug-resistant strains of Mycobacterium tuberculosis underscores the need of continuous developments on new and efficient methods to determine the susceptibility of isolates of M. tuberculosis in the search for novel antimicrobial agents. Natural products constitute an important source of new drugs, but design and implementation of antimycobacterial susceptibility testing methods are necessary for evaluate the different extracts and compounds. A number of biological assay methodologies are in current use, ranging from the classical disk diffusion and broth dilution assay format, to radiorespirometric (BACTEC), dye-based, and fluorescent/luminescence reporter assays. This review presents an analysis on the in vitro susceptibility testing methods developed for determinate antitubercular activity in natural products and related compounds (semi-synthetic natural products and natural products-derived compounds) and the criteria to select the adequate method for determination of biological activity of new natural products.

Keywords: M. tuberculosis; Natural products; Susceptibility testing.

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