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. 2010 Oct;41(4):978-83.
doi: 10.1590/S1517-838220100004000016. Epub 2010 Dec 1.

Virulence and cytotoxicity of seafood borne Aeromonas hydrophila

Seethalakshmi Illanchezian  1 , Sathishkumar Jayaraman, Muthu Saravanan Manoharan, Saritha Valsalam
Affiliations

Virulence and cytotoxicity of seafood borne Aeromonas hydrophila

Seethalakshmi Illanchezian et al. Braz J Microbiol. 2010 Oct.

Abstract

The present study was conducted to determine the virulence and cytotoxicity of Aeromonas hydrophila strains isolated from seafood samples collected from 5 major fish markets in Chennai, Tamil Nadu, India. Among 73 A. hydrophila strains isolated from fish and shrimp samples, 86.3% exhibited haemolysis, 78.1% produced slime, 98.63% produced protease and also demonstrated cytotoxicity on Vero cells. Cell shrinkage, detachment and rounding of Vero cells were recorded as cytotoxic changes. Only one strain did not show haemolysis, slime production, proteolytic activity and cytotoxicity on treatment with Vero cells. Positive correlation was observed between proteolytic activity and cytotoxicity irrespective of haemolytic activity of the strains. These results demonstrated the presence of wide spread, pathogenically characterized, cytotoxic seafood borne A. hydrophila in Chennai.

Keywords: Aeromonas hydrophila; Vero cells; cytotoxicity; seafood; virulence.

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References

    1. Abdullah A.I., Hart C.A., Winstanley C. Molecular characterization and distribution of virulence – associated genes amongst Aeromonas isolates from Libya. J Appl Microbiol. 2003;95:1001–1007. - PubMed
    1. Breneder R., Janda J.M. Detection, quantification and stability of the β – haemolysin of Aeromonas spp. J Med Microbiol. 1987;24:247–251. - PubMed
    1. Castro-Escarpulli G., Figueras M.J., Aguilera-Arreola G., Soler L., Fernandez-Rendon E., Aparicio G.O., Guarro J., Chacon M.R. Characterization of Aeromonas species isolated from frozen fish intended for human consumption in Mexico. Int J Food Microbiol. 2003;84(1):41–49. - PubMed
    1. Charney J., Tomarelli R.M. A colorimetric method for the determination of the proteolytic activity of duodenal juice. J Biol Chem. 1947;171:501–505. - PubMed
    1. Darlington L.G., Stone T.W. Antioxidants and fatty acids in the amelioration of rheumatoid arthritis and related disorders. Br J Nutr. 2001;85:251–269. - PubMed

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