Some optimal culture conditions for production of cyclosporin a by Fusarium roseum

Abstract

A suitable chemically defined culture medium was selected and some optimal conditions for the production of the highly immunosuppressive compound, cyclosporin A (Cyc A) are reported. Medium of the following composition was favorable for the production of Cyc A by Fusarium roseum: glucose, 30; NaNO3, 2; KH2PO4, 1; MgSO4.7H2O, 0.5 and KCL, 0.5 (g/l). Maximum productivity of Cyc A was achieved at pH 6.0 when 50 ml of the fermentation medium/250 ml flask, inoculated with five fungal agar discs (6 mm, diameter) of 7-days old F. roseum culture after incubation at 30 ºC at 120 rpm for 7 days.

Keywords: Cyclosporin A; Fermentation conditions; Fusarium roseum.

Figure 1

Structure of cyclosporin A as established by Dreyfuss et al. (12)

Figure 2

Effects of different fermentation periods on the production of Cyc A using F. roseum.

Figure 3

Cyc A production by F. roseum grown under different incubation temperatures.

Figure 4

Effects of initial pH on the production of Cyc A by F. roseum.

Figure 5

Cyc A production by F. roseum grown under different volumes of cultivation medium.

Figure 6

Effects of glucose concentrations on cell growth and Cyc A production. The media contained 0.5 % (w/v) of yeast extract as a nitrogen source.

Figure 7

Effects of NaNO₃ concentrations on cell growth and Cyc A production. The media contained 3.0 % (w/v) of glucose as a carbon source.