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. 2011 Jul;42(3):1109-18.
doi: 10.1590/S1517-838220110003000032. Epub 2011 Sep 1.

Production and characterization of PHA from recombinant E. coli harbouring phaC1 gene of indigenous Pseudomonas sp. LDC-5 using molasses

V Saranya  1 , R Shenbagarathai
Affiliations

Production and characterization of PHA from recombinant E. coli harbouring phaC1 gene of indigenous Pseudomonas sp. LDC-5 using molasses

V Saranya et al. Braz J Microbiol. 2011 Jul.

Abstract

Polyhydroxyalkanoates (PHA) are biodegradable and biocompatible green thermoplastics, synthesized by wide variety of bacteria as an intracellular carbon and energy storage intermediate. They are used as an alternative to nonrenewable petroleum derived plastics. The current interest in these biopolyesters is stimulated by the search for cost-effective capitalized production. This paper attempts to achieve maximized production rate from recombinant system using inexpensive substrate. Molasses from agro-industrial waste was used to produce PHA from recombinant E.coli in batch culture. PHA yield in molasses (3.06g/L ± 0.05‒75.5%) was higher than that of sucrose (2.5g/L ± 0.05 - 65.1%). Properties of the polymer produced from molasses and sucrose were analyzed by DSC, TGA, DTA, GC/MS, TLC and optical rotation studies. The findings suggested that molasses enhanced PHA production in recombinant E.coli.

Keywords: Polyhydroxyalkanoates; cost effective production; molasses.

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Figures

Figure 1
Figure 1
Phase contrast microscopic view of recombinant E.coli harboring partial phaC1 gene a. Recombinant E.coli grown for 12hin Sucrose b. Recombinant E.coli grown for 72hin Sucrose c. Recombinant E.coli grown for 12hin Molasses d. Recombinant E.coli grown for 72hin Molasses
Figure 2
Figure 2
Growth kinetics of recombinant E.coli a. PHA production per Litre by recombinant E.coli grown using Sucrose b. PHA production per Litre by recombinant E.coli using Molasses CDW – Cell Dry Weight ; PHA – Polyhydroxyalkanoate; RCW – Residual Cell Weight
Figure 3
Figure 3
Economic analysis: Production of one gram of PHA
Figure 4
Figure 4
Thermal analysis: Melting temperature determination from DSC thermogram a. PHA produced from Recombinant E.coli by fermentation of molasses b. PHA produced from Recombinant E.coli by fermentation of sucrose
Figure 5
Figure 5
TGA and DTA thermogram for determination of Decomposition and curing Temperature a. PHA produced from Recombinant E.coli by fermentation of molasses
Figure 5
Figure 5
TGA and DTA thermogram for determination of Decomposition and curing Temperature b. PHA produced from Recombinant E.coli by fermentation of sucrose
Figure 6
Figure 6
GC/MS analysis of PHA produced from Recombinant E.coli by fermentation of molasses
Figure 7
Figure 7
GC/MS analysis of PHA produced from Recombinant E.coli by fermentation of sucrose
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