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. 2012 Jan;43(1):62-9.
doi: 10.1590/S1517-83822012000100007. Epub 2012 Jun 1.

Production of inulinase from Kluyveromyces marxianus using dahlia tuber extract

Sumat Chand Jain  1 , P C Jain, Naveen Kango
Affiliations

Production of inulinase from Kluyveromyces marxianus using dahlia tuber extract

Sumat Chand Jain et al. Braz J Microbiol. 2012 Jan.

Abstract

Various carbon sources were evaluated for production of inulinase by yeast, Kluyveromyces marxianus MTCC 3995. Highest inulinase activity was observed with Dahlia extract (25.3 nkat mL(-1)) as carbon source. The enzyme activity was 1.4 folds higher than that observed in media containing pure chicory inulin (17.8 nkat mL(-1)). The yeast showed good growth on a simple medium containing dahlia extract (20% w/v) and yeast extract (2%w/v) as carbon and nitrogen source respectively, in 96 h. at 28°C and 120 rpm. Lowest inulinase yield (4.8 nkat mL(-1)) was seen in the medium containing glucose as C-source. Although varied inulinase levels were noticed on different C- sources, Inulinase: Sucrase (I/S) ratios were noticed to be similar. Among various protein sources tested, yeast extract was found to be the best source followed by beef extract (17.9 nkat mL(-1)) and peptone (13.8 nkat mL(-1)). The enzyme was optimally active at pH (4.0) and 50°C. TLC analysis of end product revealed that inulinase hydrolyzed inulin exclusively into fructose. Results suggest that the dahlia extract induced exoinulinase synthesis in Kluyveromyces marxianus and can be utilized as a potential substrate for inulinase production.

Keywords: Inulinase; Kluyveromyces marxianus; dahlia; fructose.

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Figures

Figure 1
Figure 1
Effect of carbon source on inulinase production by K. marxianus MTCC 3995. Culture condition: 28°C, 120 rpm; 96 h. Results represent mean ± S.D. of three experiments.
Figure 2
Figure 2
Production of inulinase by K. marxianus MTCC 3995 on dahlia yeast extract medium. Inulinase (•), pH (- -) and biomass (▲). Results represent average of three independent experiments.
Figure 3
Figure 3
Effect of pH on activity of inulinase of K. Marxianus MTCC 3995.
Figure 4
Figure 4
Effect of temperature on activity of inulinase of K. Marxianus MTCC 3995.
Figure 5
Figure 5
Effect of carbon source on inulinase production by K. marxianus MTCC 3995. Media contained dahlia extract (20% w/v) + nitrogen source (2% w/v). Results represent mean ± S.D. of three experiments.
Figure 6
Figure 6
Induction of inulinase activity by different carbon sources. Line1: Standards (F-Fructose, R-Raffinose), Line 2: Chicory Inulin; Line 3, 5, 7, 9 and 11 culture filtrates of K. marxianus MTCC 3995 grown on fructose, glucose, sucrose, inulin and dahlia extract media. Line 4, 6, 8, 10, and 12 end product of inulinase activity in the respective culture filtrates.
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