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. 2012 Oct;43(4):1414-8.
doi: 10.1590/S1517-838220120004000024. Epub 2012 Jun 1.

Inhibition of Listeria monocytogenes in minas frescal cheese by free and nanovesicle-encapsulated nisin

Patrícia da Silva Malheiros  1 , Daniel Joner Daroit, Adriano Brandelli
Affiliations

Inhibition of Listeria monocytogenes in minas frescal cheese by free and nanovesicle-encapsulated nisin

Patrícia da Silva Malheiros et al. Braz J Microbiol. 2012 Oct.

Abstract

The effectiveness of free and nanovesicle-encapsulated nisin to control Listeria monocytogenes in Minas Frescal cheese was investigated. Commercial nisin was encapsulated into liposomes of partially purified soy lecithin. Free (0.1 mg/mL and 0.25 mg/mL) and nanovesicle-encapsulated nisin (0.25 mg/mL) were applied onto the surface of cheese samples, and L. monocytogenes was inoculated before incubation at 6-8°C for 28 days. A bactericidal effect was observed with 0.25 mg/mL free nisin; a bacteriostatic effect was observed for liposome-encapsulated nisin and 0.1 mg/mL free nisin. Free nisin was more efficient than nisin-loaded liposomes in controlling L. monocytogenes. Possible reasons for this behavior, and also the significance of nisin to soft cheeses are discussed. Nisin acted as a suitable barrier within hurdle technology, potentially extending the shelf-life and safety of fresh cheeses.

Keywords: Listeria monocytogenes; cheese; encapsulation; liposome; nisin.

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