Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Jan;172(1):491-4.
doi: 10.1128/jb.172.1.491-494.1990.

In vivo degradation of secreted fusion proteins by the Escherichia coli outer membrane protease OmpT

F Baneyx  1 , G Georgiou
Affiliations

In vivo degradation of secreted fusion proteins by the Escherichia coli outer membrane protease OmpT

F Baneyx et al. J Bacteriol. 1990 Jan.

Abstract

The Escherichia coli outer membrane protease OmpT (protease VII) has been shown to degrade several proteins in vitro, but its function in vivo is uncertain. We demonstrate that OmpT participates in the degradation of a fusion protein secreted into the periplasmic space. A strain with mutations in degP (K.L. Strauch and J. Beckwith, Proc. Natl. Acad. Sci. USA 85:1576-1580, 1988) and ompT exhibits a cumulative decrease in protein degradation and should be useful for the expression of proteolytically sensitive secreted proteins.

PubMed Disclaimer

References

    1. Anal Biochem. 1987 Jan;160(1):47-56 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8129-33 - PubMed
    1. Nucleic Acids Res. 1988 Feb 11;16(3):1209 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1576-80 - PubMed
    1. J Bacteriol. 1988 May;170(5):2212-20 - PubMed

Publication types

MeSH terms

LinkOut - more resources