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. 1990 Jan;10(1):426-9.
doi: 10.1128/mcb.10.1.426-429.1990.

ACE1 transcription factor produced in Escherichia coli binds multiple regions within yeast metallothionein upstream activation sequences

C F Evans  1 , D R Engelke, D J Thiele
Affiliations

ACE1 transcription factor produced in Escherichia coli binds multiple regions within yeast metallothionein upstream activation sequences

C F Evans et al. Mol Cell Biol. 1990 Jan.

Abstract

The ACE1 protein of Saccharomyces cerevisiae was expressed as a trpE-ACE1 fusion protein in Escherichia coli and shown to bind CUP1 upstream activation sequences at multiple regions in a copper-inducible manner. These binding sites contain within them the sequence 5'-TC(T)4-6GCTG-3', which we propose constitutes an important part of the ACE1 consensus recognition sequence.

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References

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