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. 2013 Sep;108(6):801-3.
doi: 10.1590/0074-0276108062013019.

Red blood cells derived from peripheral blood and bone marrow CD34⁺ human haematopoietic stem cells are permissive to Plasmodium parasites infection

Carmen Fernandez-Becerra  1 , Joel Lelievre, Mireia Ferrer, Nuria Anton, Richard Thomson, Cristina Peligero, Maria Jesus Almela, Marcus Vg Lacerda, Esperanza Herreros, Hernando A Del Portillo
Affiliations

Red blood cells derived from peripheral blood and bone marrow CD34⁺ human haematopoietic stem cells are permissive to Plasmodium parasites infection

Carmen Fernandez-Becerra et al. Mem Inst Oswaldo Cruz. 2013 Sep.

Abstract

The production of fully functional human red cells in vitro from haematopoietic stem cells (hHSCs) has been successfully achieved. Recently, the use of hHSCs from cord blood represented a major improvement to develop the continuous culture system for Plasmodium vivax. Here, we demonstrated that CD34⁺ hHSCs from peripheral blood and bone marrow can be expanded and differentiated to reticulocytes using a novel stromal cell. Moreover, these reticulocytes and mature red blood cells express surface markers for entrance of malaria parasites contain adult haemoglobin and are also permissive to invasion by P. vivax and Plasmodium falciparum parasites.

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Figures

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Phenotypic and functional analyses of red blood cells (RBCs) derived from CD34 + human haematopoietic stem cells (hHSCs) of peripheral blood (PB) and bone marrow (BM). A: differentiation of hHSCs observed on bright field after cytospin and staining with Giemsa and Brilliant cresyl blue at the indicated days. Magnitude 1,000X; B: analysis of haemoglobin by Maldi TOF Mass Spectrometry [human control RBCs (red), reticulocytes from BM CD34 + cells (blue), reticulocytes from human umbilical cord CD34 + cells (green)]; C: flow cytometry analysis of day 14 reticulocytes derived from PB CD34 + hHSCs and differentiated either with 3T3 or MS5 feeder stromal cells. Cells were double labelled with antibodies against glycophorin A (GlyA-APC) and Duffy blood receptor (Duffy-PE); D: Giemsa stained smears of RBCs derived from PB CD34 + hHSCs infected with Plasmodium falciparum (1) and Plasmodium vivax (2) after 24 h post infection.
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