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. 2013 Sep;108(6):812-3.
doi: 10.1590/0074-0276108062013022.

The antimicrobial susceptibility, biofilm formation and genotypic profiles of Staphylococcus haemolyticus from bloodstream infections

Patricia Vollú Silva  1 , Raquel Souza Cruz, Luiz Sérgio Keim, Geraldo Renato de Paula, Bernadete Teixeira Ferreira Carvalho, Leonardo Rocchetto Coelho, Maria Cícera da Silva Carvalho, Joel Mauricio Corrêa da Rosa, Agnes Marie Sá Figueiredo, Lenise Arneiro Teixeira
Affiliations

The antimicrobial susceptibility, biofilm formation and genotypic profiles of Staphylococcus haemolyticus from bloodstream infections

Patricia Vollú Silva et al. Mem Inst Oswaldo Cruz. 2013 Sep.

Abstract

We analysed the antimicrobial susceptibility, biofilm formation and genotypic profiles of 27 isolates of Staphylococcus haemolyticus obtained from the blood of 19 patients admitted to a hospital in Rio de Janeiro, Brazil. Our analysis revealed a clinical significance of 36.8% and a multi-resistance rate of 92.6% among these isolates. All but one isolate carried the mecA gene. The staphylococcal cassette chromosome mec type I was the most prevalent mec element detected (67%). Nevertheless, the isolates showed clonal diversity based on pulsed-field gel electrophoresis analysis. The ability to form biofilms was detected in 66% of the isolates studied. Surprisingly, no icaAD genes were found among the biofilm-producing isolates.

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Figures

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A: biofilms of representative isolates of Staphylococcus haemolyticus (1: untreated; 2: proteinase K; 3: DNase; 4: sodium metaperiodate); B: microtitre based biofilm of representative isolates (1, 2: 33/08; 3, 4: 37/08; 5, 6: 69/08; 7, 8: ica -dependent biofilm producer Staphylococcus epidermidis 70D); even numbers: proteinase K treatment; odd numbers: untreated.
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