Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013:8:3427-35.
doi: 10.2147/IJN.S49745. Epub 2013 Sep 6.

Carbon nanoparticles downregulate expression of basic fibroblast growth factor in the heart during embryogenesis

Mateusz Wierzbicki  1 , Ewa Sawosz, Marta Grodzik, Anna Hotowy, Marta Prasek, Sławomir Jaworski, Filip Sawosz, André Chwalibog
Affiliations

Carbon nanoparticles downregulate expression of basic fibroblast growth factor in the heart during embryogenesis

Mateusz Wierzbicki et al. Int J Nanomedicine. 2013.

Abstract

Carbon nanoparticles, with their high biocompatibility and low toxicity, have recently been considered for biomedical applications, including antiangiogenic therapy. Critical to normal development and tumor formation, angiogenesis is the process of forming capillary blood vessels from preexisting vessels. In the present study, we evaluated the effects of diamond and graphite nanoparticles on the development of chicken embryos, as well as vascularization of the chorioallantoic membrane and heart at the morphological and molecular level. Nanoparticles did not affect either body/heart weight or serum indices of the embryos' health. However, vascularization of the heart and the density of branched vessels were significantly reduced after treatment with diamond nanoparticles and, to a lesser extent, graphite nanoparticles. Application of nanoparticles significantly downregulated gene and protein expression of the proangiogenic basic fibroblast growth factor, indicating that both diamond and graphite nanoparticles inhibit angiogenesis.

Keywords: VEGF; bFGF; diamond; graphite; nanoparticles; vasculogenesis.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Transmission electron microscopy images of nanoparticles. Note: Images of ND (A and B) and GR (C and D). Abbreviations: ND, diamond nanoparticles; GR, graphite nanoparticles.
Figure 2
Figure 2
CAM vascularization. Notes: Filter paper implants: (A) control group; (B) ND-treated group; and (C) GR-treated group. Gelatin sponges: (D) control group; (E) positive control with bFGF; (F) diamond nanoparticles-treated group; and (G) GR-treated group. Representative vessels of control CAM (A) and after the administration of (B) ND and (C) GR. Abbreviations: CAM, chorioallantoic membrane; ND, diamond nanoparticles; GR, graphite nanoparticles; bFGF, basic fibroblast growth factor.
Figure 3
Figure 3
Visualization of CAM vascularization in 11-day-old chicken embryo. Notes: Experimental solutions were administered in ovo by injection of 0.3 mL PBS containing hydrocolloids of ND or GR (5,000 μg/mL) into the air sack. (A) Control CAM and CAM after the administration of (B) ND or (C) GR. Agglomerates of ND located around vessels (D). Abbreviations: CAM, chorioallantoic membrane; PBS, phosphate buffered saline; ND, diamond nanoparticles; GR, graphite nanoparticles.
Figure 4
Figure 4
Images of chicken embryo heart. Notes: Experimental solutions were administered in ovo by injection of 0.3 mL PBS containing 5,000 μg/mL hydrocolloids of nanoparticles into the air sack. (A) Control heart and after the administration of (B) ND or (C) GR. Abbreviations: CAM, chorioallantoic membrane; PBS, phosphate buffered saline; ND, diamond nanoparticles; GR, graphite nanoparticles.
See this image and copyright information in PMC

References

    1. Schrand AM, Ciftan SA, Shenderova OA. Nanodiamond particles: properties and perspectives for bioapplications. Crit Rev Solid State Mat Sci. 2009;34(1–2):18–74.
    1. Huang H, Pierstorff E, Liu K, Ōsawa E, Ho D. Nanodiamond-mediated delivery of therapeutics via particle and thin film architectures. In: Ho D, editor. Nanodiamonds: Applications in Biology and Nanoscale Medicine. New York: Springer: Science+Business Media; 2010. pp. 151–174.
    1. Schrand AM, Dai L, Schlager JJ, Hussain SM, Ōsawa E. Differential biocompatibility of carbon nanotubes and nanodiamonds. Diamond Relat Mater. 2007;16(12):2118–2123.
    1. Liu KK, Chen MF, Chen PY, et al. Alpha-bungarotoxin binding to target cell in a developing visual system by carboxylated nanodiamond. Nanotechnology. 2008;19(20):205102. - PubMed
    1. Thomas V, Halloran BA, Ambalavanan N, Catledge SA, Vohra YK. In vitro studies on the effect of particle size on macrophage responses to nanodiamond wear debris. Acta Biomater. 2012;8(5):1939–1947. - PMC - PubMed

Publication types

MeSH terms