Wound-inducible nuclear protein binds DNA fragments that regulate a proteinase inhibitor II gene from potato
- PMID: 2405385
- PMCID: PMC53313
- DOI: 10.1073/pnas.87.2.603
Wound-inducible nuclear protein binds DNA fragments that regulate a proteinase inhibitor II gene from potato
Abstract
Deletion analysis from the 3' to the 5' end of the promoter region of the wound-inducible potato proteinase inhibitor IIK gene has identified a 421-base sequence at -136 to -557 that is necessary for expression. Utilizing DNA band-shift assays, a 10-base sequence within the 421-base region was found to bind a nuclear protein from wounded tomato leaves. This 10-base sequence is adjacent to an 8-base consensus sequence at -147 to -155 that is present in the promoter region of several elicitor-inducible genes from various other plants. The evidence suggests that a complex set of cis- and trans-acting elements within the -136 to -165 region of the potato IIK gene may be involved with the signaling mechanisms that regulate the inducibility of this gene in response to pest and pathogen attacks.
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