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. 2013:2013:913135.
doi: 10.1155/2013/913135. Epub 2013 Aug 26.

Transforming growth factor β1 genotypes in relation to TGFβ1, interleukin-8, and tumor necrosis factor alpha in induced sputum and blood in cystic fibrosis

O Eickmeier  1 , L v D Boom, F Schreiner, M J Lentze, D NGampolo, R Schubert, S Zielen, S Schmitt-Grohé
Affiliations

Transforming growth factor β1 genotypes in relation to TGFβ1, interleukin-8, and tumor necrosis factor alpha in induced sputum and blood in cystic fibrosis

O Eickmeier et al. Mediators Inflamm. 2013.

Abstract

Background: High-producer TGFβ1 genotypes are associated with severe lung disease in cystic fibrosis (CF), but studies combining IL-8, TNFα-, and TGFβ1(+genotype) levels and their impact on CF lung disease are scarce.

Aim: Assessing the relationship between TGF β 1, IL-8, and TNF- α and lung disease in CF in an exacerbation-free interval.

Methods: Twenty four patients delta F508 homozygous (median age 20.5 y, Shwachman score 75, FEV1(%) 83) and 8 controls (median age 27.5 y) were examined. TGF β 1 was assessed in serum and induced sputum (IS) by ELISA, for IL-8 and TNF- α by chemiluminescence in IS and whole blood. Genotyping was performed for TGF β 1 C-509T and T+869C utilizing RFLP.

Results: TGF β 1 in IS (CF/controls median 76.5/59.1 pg/mL, P < 0.074) was higher in CF. There was a negative correlation between TGF β 1 in serum and lung function (LF) (FEV1 (r = -0.488, P = 0.025), MEF 25 (r = -0.425, P = 0.055), and VC (r = -0.572, P = 0.007)). Genotypes had no impact on TGF β 1 in IS, serum, and LF. In IS TGF β 1 correlated with IL-8 (r = 0.593, P < 0.007) and TNF- α (r = 0.536, P < 0.018) in patients colonized by bacteria with flagellin.

Conclusion: TGF β 1 in serum not in IS correlates with LF. In patients colonized by bacteria with flagellin, TGF β 1 correlates with IL-8 and TNF- α in IS.

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Figures

Figure 1
Figure 1
Log 10 TGFβ1 (pg/mL) in induced sputum in CF patients colonized by either P. aeruginosa, S. aureus, or S. maltophilia plotted as a function of Log 10 IL-8 with linear regression line (r = 0.593, P < 0.007).
Figure 2
Figure 2
Log 10 TGFβ1 (pg/mL) in induced sputum in CF patients colonized by either P. aeruginosa, S. aureus, or S. maltophilia plotted as a function of Log 10 TNF-alpha with linear regression line (r = 0.536, P < 0.018).
Figure 3
Figure 3
Log 10 TGFβ1 (pg/mL) in serum in CF patients plotted as a function of Log FEV1 (%) with linear regression line (r = −0.488, P < 0.025).
Figure 4
Figure 4
Log 10 TGFβ1 (pg/mL) in serum in CF patients plotted as a function of Log 10 MEF 25 (%) with linear regression line (r = −0.425, P < 0.055).
Figure 5
Figure 5
Log 10 TGFβ1 (pg/mL) in serum in CF patients plotted as a function of Log VC (%) with linear regression line (r = −0.572, P < 0.007).
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