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Randomized Controlled Trial
. 2014 Jan;58(1):113-6.
doi: 10.1093/cid/cit641. Epub 2013 Sep 24.

Point-of-care diagnosis and prognostication of cryptococcal meningitis with the cryptococcal antigen lateral flow assay on cerebrospinal fluid

Taseera Kabanda  1 , Mark J Siedner, Jeffrey D Klausner, Conrad Muzoora, David R Boulware
Affiliations
Randomized Controlled Trial

Point-of-care diagnosis and prognostication of cryptococcal meningitis with the cryptococcal antigen lateral flow assay on cerebrospinal fluid

Taseera Kabanda et al. Clin Infect Dis. 2014 Jan.

Abstract

The cryptococcal antigen (CRAG) lateral flow assay (LFA) had 100% sensitivity and specificity on cerebrospinal fluid samples. Pretreatment LFA titers correlated with quantitative cultures (R(2) = 0.7) and predicted 2- and 10-week mortality. The CRAG LFA is an accurate diagnostic assay for CSF and should be considered for point-of-care diagnosis of cryptococcal meningitis.

Keywords: HIV; cryptococcal meningitis; diagnosis; point-of-care; sub-Saharan Africa.

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Figures

Figure 1.
Figure 1.
Two- and 10-week mortality by pretreatment cryptococcal antigen lateral flow assay titers. Error bars signify 1 standard deviation. P values denote results of χ2 testing between groups. Multivariable analyses are adjusted for pretreatment Glasgow Coma Scale score at diagnosis. Odds ratios are per each increase in pretreatment log10 colony-forming units per milliliter of cerebrospinal fluid culture at diagnosis, and per each 2-fold dilution >1:10 in pretreatment cryptococcal antigen lateral flow assay titer at diagnosis. Abbreviations: CFU, colony-forming unit; CI, confidence interval; CSF, cerebrospinal fluid; LFA, lateral flow assay.
See this image and copyright information in PMC

References

    1. McMullan BJ, Halliday C, Sorrell TC, et al. Clinical utility of the cryptococcal antigen lateral flow assay in a diagnostic mycology laboratory. PLoS One. 2012;7:e49541. - PMC - PubMed
    1. Hansen J, Slechta ES, Gates-Hollingsworth MA, et al. Large-scale evaluation of the immuno-mycologics lateral flow and enzyme-linked immunoassays for detection of cryptococcal antigen in serum and cerebrospinal fluid. Clin Vaccine Immunol. 2013;20:52–5. - PMC - PubMed
    1. World Health Organization. Rapid advice: diagnosis, prevention and management of cryptococcal disease in HIV-infected adults, adolescent and children. Available at: www.who.int/hiv/pub/cryptococcal_disease2011/en/ . Accessed 22 March 2013. - PubMed
    1. Boulware DR, Meya D, Muzoora C, et al. ART initiation within the first 2 weeks of cryptococcal meningitis is associated with higher mortality: a multisite randomized trial. 20th Conference on Retroviruses and Opportunistic Infections,; Atlanta, Georgia,. 3–6 March 2013.
    1. Muzoora CK, Kabanda T, Ortu G, et al. Short course amphotericin B with high dose fluconazole for HIV-associated cryptococcal meningitis. J Infect. 2012;64:76–81. - PubMed

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