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. 2013 Nov 11;49(87):10323-5.
doi: 10.1039/c3cc43389g.

Creation of a caspase-3 sensing system using a combination of split-GFP and split-intein

Seiji Sakamoto  1 , Mika Terauchi, Anna Hugo, Tanner Kim, Yasuyuki Araki, Takehiko Wada
Affiliations

Creation of a caspase-3 sensing system using a combination of split-GFP and split-intein

Seiji Sakamoto et al. Chem Commun (Camb). .

Abstract

A genetically encodable caspase-3 sensing system has been created using self-assembling split-GFP, in which a C-terminal fragment is "covalently" cyclized via a caspase-3 substrate sequence mediated by split-intein. The specific cleavage of the cyclic C-terminal fragment by caspase-3 induces the GFP reassembly and fluorescence recovery.

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