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. 2013 Dec;51(12):4226-9.
doi: 10.1128/JCM.02612-13. Epub 2013 Sep 25.

Comparison of heat inactivation and cell disruption protocols for identification of mycobacteria from solid culture media by use of vitek matrix-assisted laser desorption ionization-time of flight mass spectrometry

Alexandra Machen  1 , Miwako Kobayashi, Mary Robin Connelly, Yun F Wayne Wang
Affiliations

Comparison of heat inactivation and cell disruption protocols for identification of mycobacteria from solid culture media by use of vitek matrix-assisted laser desorption ionization-time of flight mass spectrometry

Alexandra Machen et al. J Clin Microbiol. 2013 Dec.

Abstract

Two novel protocols for inactivation and extraction were developed and used to identify 107 Mycobacterium clinical isolates, including Mycobacterium tuberculosis complex, from solid cultures using Vitek matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. The protocol using heat inactivation with sonication and cell disruption with glass beads resulted in 82.2% and 88.8% species and genus level identifications, respectively.

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Figures

Fig 1
Fig 1
Flow chart of inactivation protocols used prior to identification (ID) by Vitek MALDI-TOF MS RUO (Vitek MS). Note that the suspension spot must dry completely before adding the CHCA matrix.
Fig 2
Fig 2
Spectral fingerprints obtained by use of Vitek MS after inactivation. m/z, mass-to-charge ratio.
See this image and copyright information in PMC

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