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. 2008 Sep 20;463(1-3):145-149.
doi: 10.1016/j.cplett.2008.08.039.

Challenge in Understanding Size and Shape Dependent Toxicity of Gold Nanomaterials in Human Skin Keratinocytes

Shuguang Wang  1 , Wentong Lu, Oleg Tovmachenko, Uma Shanker Rai, Hongtao Yu, Paresh Chandra Ray
Affiliations

Challenge in Understanding Size and Shape Dependent Toxicity of Gold Nanomaterials in Human Skin Keratinocytes

Shuguang Wang et al. Chem Phys Lett. .

Abstract

As the nanotechnology field continues to develop, assessing nanoparticle toxicity is very important for advancing nanoparticles for biomedical application. Here we report cytotoxicity of gold nanomaterial of different size and shape using MTT test, absorption spectroscopy and TEM. Spherical gold nanoparticles with different sizes are not inherently toxic to human skin cells, but gold nanorods are highly toxic due to the presence of CTAB as coating material. Due to CTAB toxicity, and aggregation of gold nanomaterials in the presence of cell media, it is a real challenge to study the cytotoxicity of gold nanomaterials individually.

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Figures

Figure 1
Figure 1
Absorption spectra, photograph and TEM images of gold nanorod of different aspect ratios
Figure 2
Figure 2
Figure 2A: Viability of cells incubated with gold nanoparticle of different sizes, 2B: Time dependents absorption change in the presence DMEM of 30 nm gold nanoparticle and 2C: TEM image for gold nanoparticle in the presence of cell media.
Figure 2
Figure 2
Figure 2A: Viability of cells incubated with gold nanoparticle of different sizes, 2B: Time dependents absorption change in the presence DMEM of 30 nm gold nanoparticle and 2C: TEM image for gold nanoparticle in the presence of cell media.
Figure 3
Figure 3
Figure 3A: Viability of cells incubated with gold nanorod (GNR, aspect ratio 3.0) with CTAB, only CTAB and PSS coated GNR, 3B: Time dependents absorption change in the presence of DMEM for gold nanorod with aspect ratio 3.0 and 3C: TEM data for gold nanorod in presence of cell media.
Figure 4
Figure 4
Figure 4A: TEM data for PSS-coated gold nanorod, 4B: Viability of cells incubated with PSS-coated gold nanorod (3.2 aspect ration) of different aspect ratios
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