doi: 10.1016/0022-2836(90)90357-R.
N4 RNA polymerase II sites of transcription initiation
Affiliations
- PMID: 2407852
- DOI: 10.1016/0022-2836(90)90357-R
Item in Clipboard
N4 RNA polymerase II sites of transcription initiation
J Mol Biol.
.
Abstract
The RNA polymerase responsible for the synthesis of coliphage N4 middle RNAs, N4 RNA polymerase II, is composed of two subunits of 30,000 and 40,000 molecular weight. It is the smallest DNA-dependent RNA polymerase characterized to date. We have determined the sequences surrounding the sites of in vivo transcription initiation for this enzyme. Two regions of sequence homology are present: a box at +1, 3' AAAT 5', and a box, 3' TTCTGGAC 5' at a variable distance (16 to 24 base-pairs) upstream from +1. Possible mechanisms for recognition of these sequences are discussed.
Similar articles
-
In vitro requirements for N4 RNA polymerase II-specific initiation.J Biol Chem. 1989 Jul 25;264(21):12695-9. J Biol Chem. 1989. PMID: 2663865
-
The bacteriophage N4-coded single-stranded DNA-binding protein (N4SSB) is the transcriptional activator of Escherichia coli RNA polymerase at N4 late promoters.J Mol Biol. 1995 Mar 3;246(4):461-71. doi: 10.1006/jmbi.1994.0098. J Mol Biol. 1995. PMID: 7877167
-
Bacteriophage N4-induced transcribing activities in E. coli. III. A third cistron required for N4 RNA polymerase II activity.Virology. 1983 Apr 30;126(2):678-87. doi: 10.1016/s0042-6822(83)80023-3. Virology. 1983. PMID: 6344415
-
The transition from initiation to elongation by RNA polymerase II.Cold Spring Harb Symp Quant Biol. 1998;63:289-300. doi: 10.1101/sqb.1998.63.289. Cold Spring Harb Symp Quant Biol. 1998. PMID: 10384293 Review. No abstract available.
-
Viroids and their interactions with host cells.Annu Rev Microbiol. 1982;36:239-58. doi: 10.1146/annurev.mi.36.100182.001323. Annu Rev Microbiol. 1982. PMID: 6756289 Review. No abstract available.
Cited by
-
Phage N4 RNA polymerase II recruitment to DNA by a single-stranded DNA-binding protein.Genes Dev. 2003 Sep 15;17(18):2334-45. doi: 10.1101/gad.1121403. Genes Dev. 2003. PMID: 12975320 Free PMC article.
-
Structural and biochemical investigation of bacteriophage N4-encoded RNA polymerases.Biomolecules. 2015 Apr 27;5(2):647-67. doi: 10.3390/biom5020647. Biomolecules. 2015. PMID: 25924224 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
