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. 2013 Jul;5(3):214-23.
doi: 10.4103/0975-7406.116822.

In vitro antioxidant and H(+), K(+)-ATPase inhibition activities of Acalypha wilkesiana foliage extract

Rajesh Kashi Prakash Gupta  1 , Pradeepa, Manjunatha Hanumanthappa
Affiliations

In vitro antioxidant and H(+), K(+)-ATPase inhibition activities of Acalypha wilkesiana foliage extract

Rajesh Kashi Prakash Gupta et al. J Pharm Bioallied Sci. 2013 Jul.

Abstract

Aims: The aim of this study was to evaluate the antioxidant activty and anti-acid property of Acalypha wilkesiana foliage extract.

Materials and methods: Hot and cold aqueous extracts were prepared from healthy leaves of A. wilkesiana. Free radical scavenging activity and H(+), K(+)-ATPase inhibition activities of aqueous foliage extracts was screened by in vitro models.

Statistical analysis used: All experiments were performed in triplicate and results are expressed as mean ± SEM.

Results: A. wilkesiana hot aqueous extract (AWHE) showed significant antioxidants and free radical scavenging activity. Further, AWHE has shown a potent H(+), K(+)-ATPase inhibitory activity (IC50: 51.5 ± 0.28 μg/ml) when compare to standard proton pump inhibitor omeprazole (56.2 ± 0.64 μg/ml); however, latter activity is equal to A. wilkesiana cold aqueous extract (AWCE). Quantitative analysis of AWHE has revealed more content of phenols and flavonoids; this is found to be the reason for good antioxidant activity over AWCE. Molecular docking was carried out against H(+), K(+)-ATPase enzyme crystal structure to validate the anti-acid activity of A. wilkesiana major phytochemicals.

Conclusions: The present study indicates that the constituents of AWHE and AWCE have good antacid and free radical scavenging activity.

Keywords: Acalypha wilkesiana; Anti-acid; antioxidant; gallic acid; gastric ulcer; molecular docking; polyphenols.

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Conflict of interest statement

Conflict of Interest: The authors certify that, there is no conflict of interest.

Figures

Figure 1
Figure 1
Balance between gastro-protective and aggressive factors
Figure 2
Figure 2
Structure of H+, K+ -ATPase (PDB ID: 2XZB)
Figure 3
Figure 3
Antioxidant activity of AWHE and AWCE (a) Total antioxidant capacity, (b) total reductive capability, (c) DPPH radical scavenging activity, (d) Superoxide radical scavenging activity and (e) Lipid peroxidation inhibition activity
Figure 4
Figure 4
H+, K+-ATPase inhibition study by AWHE and AWCE in comparison to Omeprazole
Figure 5
Figure 5
Molecular docking studies of major phytoconstituent (a) artemetin, (b) corilagin, (c) gallic acid, (d) geranin, (e) kaempferol, (f) lutein, (g) luteolin, (h) quercetin, (i) vitexicarpin and (j) omeprazole against H+, K+-ATPase
See this image and copyright information in PMC

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