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. 2013 Sep 23;8(9):e74269.
doi: 10.1371/journal.pone.0074269. eCollection 2013.

High performance of histidine-rich protein 2 based rapid diagnostic tests in French Guiana are explained by the absence of pfhrp2 gene deletion in P. falciparum

Mélanie Trouvay  1 , Georges Palazon, Franck Berger, Béatrice Volney, Denis Blanchet, Emilie Faway, Damien Donato, Eric Legrand, Bernard Carme, Lise Musset
Affiliations

High performance of histidine-rich protein 2 based rapid diagnostic tests in French Guiana are explained by the absence of pfhrp2 gene deletion in P. falciparum

Mélanie Trouvay et al. PLoS One. .

Abstract

Background: Care for malaria patients in endemic areas has been improved through the increasing use of Rapid Diagnostic Tests (RDTs). Most RDTs target the histidine-rich protein-2 antigen (PfHRP2) to detect P. falciparum, as it is abundant and shows great heat stability. However, their use in South America has been widely questioned following a recent publication that pinpoints the high prevalence of Peruvian field isolates lacking the gene encoding this protein. In the remote rural health centers of French Guiana, RDTs are the main diagnosis tools. Therefore, a study of PfHRP2 RDT performances and pfhrp2 genotyping was conducted to determine whether a replacement of the current pLDH-based kit could be considered.

Methods: The performance study compared the SD Malaria Ag test P.f/Pan® kit with the current gold standard diagnosis by microscopy. The prevalence of pfhrp2 and pfhrp3 deletions were evaluated from 221 P. falciparum isolates collected between 2009 and 2011 in French Guiana.

Results: Between January 2010 and August 2011, 960 suspected cases of malaria were analyzed using microscopy and RDTs. The sensitivity of the SD Malaria Ag test P.f/Pan® for detection of P. falciparum was 96.8% (95% CI: 90.9-99.3), and 86.0% (95% CI: 78.9-91.5) for the detection of P. vivax. No isolates (95% CI: 0-4.5) lacking either exon of the pfhrp2 gene were identified among the 221 P. falciparum isolates analyzed, but 7.4% (95% CI: 2.8-15.4) lacked the exon 2 part of the pfhrp3 gene.

Conclusions: Field isolates lacking either exon of the pfhrp2 gene are absent in this western part of South America. Despite its sensibility to detect P. vivax, the SD Malaria Ag test P.f/Pan® kit is a satisfying alternative to microscopy in remote health centers, where it is difficult to provide highly skilled microscopists and to maintain the necessary equipment.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Map of French Guiana, part of Amazonia, South America. Figures in brackets are the number of isolates collected in each health care institution then analysed retrospectively to evaluate the prevalence of pfhrp2 deletion in French Guiana.
See this image and copyright information in PMC

References

    1. WHO (2009) Malaria rapid diagnostic test performance: results of WHO product testing of malaria RDTs: round 1 (2008). In: Organization WaH, editor. Geneva: World and Health Organization. Available: http://www.who.int/tdr/publications/documents/executive-summary-malaria-.... Accessed 1st February 2013.
    1. WHO (2010) Malaria rapid diagnostic test performance: results of WHO product testing of malaria RDTs: round 2 (2009). In: Organization WaH, editor. Geneva: World and Health Organization. Available: http://www.who.int/tdr/publications/documents/rdt_round2.pdf. Accessed 1st February 2013.
    1. WHO (2011) Malaria rapid diagnostic test performance: results of WHO product testing of malaria RDTs: round 3 (2010–2011). In: Organization WaH, editor. Geneva: World and Health Organization. Available: http://www.who.int/tdr/publications/documents/rdt3.pdf. Accessed 1st February 2013.
    1. WHO (2012) Malaria rapid diagnostic test performance: results of WHO product testing of malaria RDTs: round 4 (2012). In: Organization WaH, editor. Geneva: World and Health Organization. Available: http://apps.who.int/iris/bitstream/10665/77748/1/9789241504720_eng.pdf. Accessed 1st February 2013.
    1. WHO (2011) Good practices for selecting and procuring rapid diagnostic tests for malaria. In: Organization WaH, editor. Geneva: World and Health Organization. Available: http://whqlibdoc.who.int/publications/2011/9789241501125_eng.pdf. Accessed 1st February 2013.

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