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. 2013 Dec;3(12):936-41.
doi: 10.1016/S2221-1691(13)60182-3.

Screening and characterization of extracelluar L-asparaginase producing Bacillus subtilis strain hswx88, isolated from Taptapani hotspring of Odisha, India

Biswaprakash Pradhan  1 , Sashi K Dash, Sabuj Sahoo
Affiliations

Screening and characterization of extracelluar L-asparaginase producing Bacillus subtilis strain hswx88, isolated from Taptapani hotspring of Odisha, India

Biswaprakash Pradhan et al. Asian Pac J Trop Biomed. 2013 Dec.

Abstract

Objective: To screen and isolate an eco-friendly, a thermophilic and potent L-asparaginase producing bacterium, with novel immunological properties that may obviates hypersensitivity reactions.

Methods: In the present study bacterial strain isolated for extracellular L-asparaginase production from hotspring, identified by morphological, biochemical and physiological tests followed by 16S rDNA technology and the L-asparaginase production ability was tested by both semi quantitative and quantitative enzymatic assay.

Results: The bacterial strain was identified as Bacillus subtilis strain hswx88 (GenBank Accession Number: JQ237656.1). The extracellular enzyme yielding capacity isolate Bacillus subtilis strain hswx88 (23.8 IU/mL) was found to be 1.7 and 14.5 times higher than the reference organism Pectobacterium carotovorum MTCC 1428 (14.2 IU/mL) and Bacillus sp. BCCS 034 (1.64 IU/mL).

Conclusion: The isolate is eco-friendly and useful to produce bulk quantity of extracellular, thermophilic L-asparaginase for the treatment of various tumor cases and for preparation of acrylamide free fry food preparation.

Keywords: 16S rDNA; Bacillus subtilis strain hswx88; L-asparaginase; Thermophilic bacteria.

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Conflict of interest statement

Conflict of interest statement: We declare that we have no conflict of interest.

Figures

Figure 1.
Figure 1.. Gram staining photomicrograph of TPS-5.
Figure 2.
Figure 2.. Scanning electron microscopy (SEM) of TPS-5.
Figure 3.
Figure 3.. Developed prominent pink colur surrounding the microorganism growing in modified M9 medium.
Figure 4.
Figure 4.. Phylogenetic tree of TPS-5 using neighbour joining method.
See this image and copyright information in PMC

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