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. 2013 Oct 5;4(1):39.
doi: 10.1186/2049-1891-4-39.

Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period

Yang Wang #  1 , Chao Wang #  1 , Zhuocheng Hou  2 , Kai Miao  1 , Haichao Zhao  1 , Rui Wang  1 , Min Guo  1 , Zhonghong Wu  3 , Jianhui Tian  1 , Lei An  1
Affiliations

Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period

Yang Wang et al. J Anim Sci Biotechnol. .

Abstract

The endometrium of sheep consists of plenty of raised aglandular areas called caruncular (C), and intensely glandular intercaruncular areas (IC). In order to better understand the endometrium involved mechanisms of implantation, we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period, and then compared the proteomic profiles between these two areas. We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively. By comparing the proteome of these two areas, we found 170 differentially expressed proteins (DEPs) (P < 0.05), functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue, cell adhesion and protein transport, and so on. Our study, for the first time, provided a proteomic reference for elucidating the differences between C and IC areas, as an integrated function unit respectively, during the peri-implantation period. The results could help us to better understand the implantation in the ewes. In addition, we established a relatively detailed protein database of ovine endometrium, which provide a unique reference for further studies.

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Figures

Figure 1
Figure 1
Experimental design of the study.
Figure 2
Figure 2
The endometrium of Chinese Small Tail Han ewes. Black arrows pointed to C areas, white arrows pointed to IC areas.
Figure 3
Figure 3
Hierarchical cluster analysis of all the proteins identified in different areas, biological replicates and technical replicates.
Figure 4
Figure 4
Distribution of differentially expressed proteins with different fold change in the comparison between C and IC areas. “*” means these proteins specifically expressed in C or IC areas.
Figure 5
Figure 5
Function annotation clustering results with differentially expressed proteins between C and IC areas based on GO biological process.
Figure 6
Figure 6
Significantly enriched GO categories (P < 0.01) using differentially expressed proteins that were up-regulated in C areas.
Figure 7
Figure 7
Significantly enriched GO categories (P < 0.01) using differentially expressed proteins that were up-regulated in IC areas.
See this image and copyright information in PMC

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