Abundance of class 1-3 integrons in South Carolina estuarine ecosystems under high and low levels of anthropogenic influence

Abstract

The impact of human activity on the spread of antibiotic resistant bacteria throughout coastal estuarine ecosystems is not well characterized. It has been suggested that laterally transferred genetic agents, such as integrons, play a role in the spread of resistant bacteria throughout ecosystems. This study compares the distribution of three integron classes throughout a coastal estuarine ecosystem. To determine integron distribution patterns, DNA was extracted from sediment and water collected at seven sites throughout two estuaries with different levels of anthropogenic input and integrons analyzed using quantitative PCR. The data show that while all three integron classes are present, the relative abundance is different, with class 2 integrons significantly elevated in areas of high anthropogenic input and class 1 integrons elevated in areas of low input. Our results provide a foundation for using integron gene distribution as a biomarker of urban impact on antibiotic resistance gene flow and ecosystem health.

Keywords: Antibiotic resistance; Integrase; Integrons; Urbanization; Wastewater treatment.