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. 2013 Sep 30;8(9):e75516.
doi: 10.1371/journal.pone.0075516. eCollection 2013.

Distribution of mosquitoes in the south east of Argentina and first report on the analysis based on 18S rDNA and COI sequences

Leonardo M Díaz-Nieto  1 , Arnaldo Maciá, Gustavo Parisi, Juan L Farina, María E Vidal-Domínguez, M Alejandra Perotti, Corina M Berón
Affiliations

Distribution of mosquitoes in the south east of Argentina and first report on the analysis based on 18S rDNA and COI sequences

Leonardo M Díaz-Nieto et al. PLoS One. .

Abstract

Although Mar del Plata is the most important city on the Atlantic coast of Argentina, mosquitoes inhabiting such area are almost uncharacterized. To increase our knowledge in their distribution, we sampled specimens of natural populations. After the morphological identification based on taxonomic keys, sequences of DNA from small ribosomal subunit (18S rDNA) and cytochrome c oxidase I (COI) genes were obtained from native species and the phylogenetic analysis of these sequences were done. Fourteen species from the genera Uranotaenia, Culex, Ochlerotatus and Psorophora were found and identified. Our 18S rDNA and COI-based analysis indicates the relationships among groups at the supra-species level in concordance with mosquito taxonomy. The introduction and spread of vectors and diseases carried by them are not known in Mar del Plata, but some of the species found in this study were reported as pathogen vectors.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Sampling area and freshwater habitats from General Pueyrredon municipality.
1, Urban area with natural and artificial containers; 2, roads towards Mar del Plata with puddles alongside; 3 roads in coastal area with puddles alongside; 4, area of nature reserve with wetlands, freshwater swamps and bogs.
Figure 2
Figure 2. Surveyed mosquito habitats.
A, Eryngium horridum ; B, detail of leaf axils of E . horridum ; C, freshwater pool in nature reserve; D, puddles along roads; E-G, artificial containers. Dr. Maciá has given written informed consent, as outlined in the PLOS consent form, to publication of this photograph.
Figure 3
Figure 3. Phylogenetic estimation by Maximum Likelihood.
A, Using 18S rDNA; B, COI coding-sequence. 500 replicates have been used in the estimation. Arrows indicates local mosquito species.
See this image and copyright information in PMC

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