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. 1985 Aug 19;188(1):33-6.
doi: 10.1016/0014-5793(85)80869-3.

Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid

A Dautigny, P M Alliel, L d'Auriol, D Pham Dinh, J L Nussbaum, F Galibert, P Jollès
Free article

Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid

A Dautigny et al. FEBS Lett. .
Free article

Abstract

A cDNA library from rat brain was constructed in pBR322 and screened with a 14-mer mixed oligonucleotide probe based on residues 231-235 of bovine proteolipid (PLP). A positive clone was isolated: it contained a 1334-base-pair cDNA insert and was subjected to DNA sequence analysis. The cDNA encoded information for the 276 amino acids of rat PLP. Comparison with bovine PLP sequence showed a complete amino acid sequence homology except for 4 amino acid residues.

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